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Biophysics brings quantitative, experimental and theoretical approaches from physics with biological questions and hypotheses, to study problems at and across a range of biological length scales and to understand biological systems. APPLICATION OF BIOPHYSICS AND BIOENGINEERING TO THE ASSESSMENT OF SKIN BARRIER FUNCTION QUAN YANG A thesis submitted for the degree of Doctor of Philosophy University of Bath Department of Pharmacy and Pharmacology September 2011 COPYRIGHT Attention is drawn to the fact that copyright of this thesis rests with its author. This includes development and use of, for example, new approaches to spectroscopy, microscopy, imaging, scattering and cell manipulation. 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